Journal Of Biology Education Research (JBER)

Identification of Salmonella sp. bacteria Using ISO 6579-1:2017 Method for Frozen Salmon (Salmo salar) Products

Annisa Wulan Agus Utami — 2025-05-27

Salmon is a type of marine fish from the Salmonidae family that lives in the Atlantic Ocean, both on the northern coast of America and Europe, as well as the Pacific Ocean. Salmonella sp. being one of the pathogenic bacteria that causes human disease through food and drink. Contamination can occur at any stage of the food production, distribution and consumption chain. Tests carried out to identify Salmonella bacterial contamination use the ISO 6579-1:2017 test method. This test consists of pre-enrichment, enrichment, plating out and biochemical tests. Identification of Salmonella sp bacteria on frozen salmon products at the BUSKIPM Quality Microbiology Laboratory using the ISO 6579-1:2017 standard test method showing negative test results for Salmonella sp. The reading of the results of the confirmation test carried out did not meet the specific requirements for Salmonella sp. bacteria.

Analysis of Understory Vegetation in the Pusat Suaka Satwa Elang Jawa Area

Aip Muhamad Irpan — 2025-05-27

Understory vegetation is the basic vegetation of the forest which can include grasses, shrubs, herbs and ferns which make an important contribution to ecological function and biodiversity. It contributes to nutrient cycling, soil stabilization, and forest regeneration. This study aims to analyze the structure of vegetation composition and diversity of understory plants in the Pusat Suaka Satwa Elang Jawa (PSSEJ). The research method uses square transects by determining the research location by purposive sampling. In each square transect, a plot measuring 2.5 m x 2.5 m was made. The number of plots made was 36 plots. The understory plants found were identified by cross-checking with the iNaturalist, PlantNet, Picture This - Plant identifier identification applications. Based on the results of the study, 52 species were found spread across 50 genera and 27 families. The dominant families are the Poaceae and Solanaceae families. The highest importance value index of Pseudechinolaena polystachya from the Poaceae family. The understory plant diversity index with a moderate category. It can be concluded that high species abundance does not always correlate with high INP values.

Development of Research-Based Modules in Hydroponic Learning

Mellisa — 2025-05-27

Hydroponics is an increasingly popular cultivation method due to its land and water use efficiency. To support students' understanding in this field, a research-based learning module is needed to ensure that the material presented is relevant and evidence-based. An interactive and research-based learning module can enhance students' conceptual understanding and skills in effectively applying hydroponic techniques. This study aims to develop a hydroponic module based on research results. This study utilizes the ADDIE model in the module development phase, students' needs and curriculum requirements were identified to align the module content with learning objectives. The design phase involved structuring the module by integrating recent research findings in hydroponics, selecting appropriate instructional strategies, and incorporating interactive elements. In the development phase, the module was created with interactive features and media to enhance engagement and comprehension. The implementation phase included pilot testing with students to gather feedback on usability and effectiveness. Finally, in the evaluation phase, expert validation and student assessments were conducted to measure the module's validity and effectiveness. Trials and validation were conducted by experts, including media specialists, subject matter experts, and hydroponic practitioners, to ensure the module met quality standards. Validation results indicated that the developed module was categorized as highly valid. Additionally, based on student assessments, the module received in the "good" category, demonstrating its suitability for hydroponics courses. These findings suggest that the research-based hydroponics module is an effective learning resource for students, providing both theoretical knowledge and practical applications in hydroponics education.

Lichen Pseudocyphellaria Mapping in Cibodas Botanical Garden Conservation Park

Lilis Supratman — 2025-05-27

Lichens are symbiotic organisms between fungi and algae. In Indonesia, lichen inventory research is very limited. Several Lichen families have entered critical levels such as Lobariaceae. The purpose of this study was to: record and collect the Lobariaceae family as a rare lichen, analyze population data of the Lobariaceae family, growth percentage and development of Lobariaceae family transplants. This research method used exploration with calculations based on the surface area of tree trunks, the number and coverage of thallus, and thallus coverage per square meter for each tree. The results of this study found 340 Pseudocyphellaria thallus consisting of Pseudocyphellaria sp. P. aurata, P. crocata and P. argyracea. During one year of Pseudocyphellaria transplantation, there was no growth in terms of increasing thallus cover. The development that occurred was that the transplant was able to attach well to the substrate. It is estimated that Pseudocyphellaria thallus takes longer to adapt well to the substrate to grow and develop. The conclusion of this study is that the transplanted lichen showed satisfactory growth, which indicates that this method can be used for the conservation of threatened lichen species.

TPACK-Integrated Zoology Vertebrate Booklet: Expert Validation of a Print Media Innovation Supporting Digital Learning

Nurul Fauziah — 2025-05-27

The development of technology in the field of education requires innovation in the development of learning media that are not only print-based but also able to integrate pedagogical and technological aspects. However, many printed learning media are less than optimal in supporting digital learning. Therefore, this study aims to describe the validity value of the TPACK integrated vertebrate zoology booklet as an innovative learning media that supports the integration of technology in biology education. This type of research uses the Plomp development model which consists of three stages, namely preliminary research, prototype making, and assessment. The preliminary research stage has been carried out in previous studies, and in this follow-up study only the prototype making stage was carried out. The assessment stage will be carried out in the next survey. Validation was carried out by three expert validators, namely media experts, material experts, and learning experts. The validation results from media experts, material experts, and learning experts for this learning media are included in the valid and feasible category. In conclusion, the TPACK integrated vertebrate zoology booklet is feasible to use in learning. It is recommended to conduct further research to test the effectiveness and practicality of this TPACK integrated vertebrate zoology booklet in improving various competencies in students.

Interaction of Different Cover Types and Planting Media Combination on Nutrient Uptake, Growth, and Photosynthesis Characteristics of Kepok Bananas (Musa Paradisiaca L.) During Acclimatization

Khairunnisa — 2025-05-27

Tissue culture techniques, in general, have four stages, which include induction, multiplication, root formation, and acclimatization. The acclimatization stage is the most critical stage that plants go through after coming out of the culture bottle, and different climatic conditions exist in the greenhouse and the field. Environmental conditions such as light intensity and planting media greatly affect the growth of kepok banana seedlings during the acclimatization period. This research was conducted at the UPTD Balai Induk Hortikultura Gedung Johor Medan from June to August 2024. The research uses a Split Plot Design with two factors. The first factor is the type of cover: a 12% UV plastic cover and a 75% shade net. The second factor consists of three levels of treatment of the combination of planting media, namely soil + vermicompost (2:1), soil + cocopeat (2:1), and soil + Baked husk (2:1). 12% UV plastic produces more volume and root length than 75% shade nets. The chlorophyll a level was higher than that of chlorophyll b in all treatment interactions except in the 12% UV plastic cover treatment and the combination of soil + vermicompost planting media. Nitrogen uptake with 12% UV plastic enclosure treatment was 31.94% higher than 75% shade net. The implication of this finding is the potential to utilize vermicompost as a planting media mixture at the acclimatization stage of kepok banana.

Daily Behavior of Javan Bull (Bos javanicus javanicus) in Ragunan Wildlife Park

Afghan Nail Irfan — 2025-05-27

Indonesia has quite a variety of endemic animals. One of Indonesia's endemic animals is the Javan bull (Bos javanicus javanicus). This animal comes from the island of Java and is now a protected animal. The decline in the Javan bull population in Indonesia is caused by illegal hunting, habitat destruction and exploitation. Conservation efforts are needed to protect and preserve the Javan bull. There is ex-situ conservation as a place to preserve various types of animals with a modern concept. Ragunan Wildlife Park is an ex-situ conservation area created in a way that matches its natural habitat. The aim of this research is to describe the daily behavior of Javan bulls and differences in the percentage of behavior of each individual Javan bull (Bos javanicus javanicus) in the Ragunan Wildlife Park ex-situ captivity. This research was carried out with a total of 12 days of observation on 4 Javan bulls, namely 2 males and 2 females. Observations were divided into 2 sessions. The morning session was held at 08.00-11.00 WIB and the afternoon session was held at 13.00-15.00 WIB using the focal animal sampling method. The parameters measured are temperature and humidity. Based on the results of research that has been carried out, the behaviors of Javan bulls that emerge are eating, drinking, ruminating, moving, resting, sleeping, grooming, urinating, defecating and social behavior. The highest behavior of the Javan bull (Bejo) was social behavior at 32.56% and the lowest was defecation behavior at 0.44%. The dominant behavior of Javan bulls (Raju) is resting behavior at 39.56% and the lowest is urination behavior at 0.22%. The highest behavior of the Javan bull (Amy) was eating behavior at 35.00% and the lowest was drinking and defecation behavior at 0.33%. The highest behavior of the Javan bull (Susi) was eating behavior at 49.44% and the lowest was sleeping behavior at 0.00%.

Development of a Gastropod Diversity E-booklet as a Learning Media for High School Students

Raden Teti Rostikawati — 2025-05-27

The study of Gastropod diversity in Cibuaya is very important, since Gastropods are the most important organisms in the food chain in aquatic ecosystems. To understand Gastropods, students need electronically supported learning aids in the form of e-booklet. One of the very important roles of e-booklets students can access e-booklets anytime, anywhere on their devices. The aim of this research is to develop a research-based electronic booklet for the identification of gastropod diversity, based on the findings from the identification of gastropods at Cibuaya Beach, Sukabumi. The development method used in this research is the 4-D model, which includes definition, design, development, and dissemination. This research was realized by developing the research results into an electronic brochure on gastropod diversity at Cibuaya Beach, Ujung Genteng, Sukabumi. Validation of the e-booklet was carried out by three Lecturers who were experts in material content, language, and media, and three Biology teachers to validate regarding the content of the material which is adapted to the material at school . The results of the validation test produced an average score of 86%. This score indicates that the E-booklet learning support falls into the very viable category and can be used in learning activities.

Monitoring CO2 Levels and Total Volatile Organic Compounds in Tofu Waste Composting Process Using IoT-Based Maggot Larvae

Ahmad Zakaria — 2025-05-27

The increasing volume of waste poses significant environmental and public health risks if not properly managed. The management of unprocessed waste may be initiated at the household level by applying principles of the circular economy. This study aims to investigate the integration of bioconversion technology using Black Soldier Fly (BSF) larvae with an IoT-based air quality monitoring system for tofu dregs waste management. A microcontroller-based device was developed to monitor and control humidity, carbon dioxide (CO₂), and Total Volatile Organic Compounds (TVOCs) using sensors and an adsorption-based scrubber system. The results indicate that CO₂ levels decreased from 1470–1747 ppm to 400–483 ppm, while TVOC levels were reduced from 163–346 ppb to 0–6 ppb after treatment, significantly improving air quality and minimizing odor emissions. The combination of BSF larvae-assisted bioconversion and automated emission control demonstrates a highly effective and sustainable approach to organic waste processing, reducing environmental pollution and generating valuable byproducts for economic utilization. This system offers a scalable solution for future applications in circular economy-based waste management. In conclusion, this system offers a scalable solution for future applications in circular economy-based waste management.

Insect Diversity in Dramaga Protected Forest Area Bogor West Java

Amelia Rizqia Al Khairina — 2025-05-27

Dramaga Protected Forest is a good habitat for various fauna, including insects. Insects have a positive role as pollinators, decomposers, predators and environmental bioindicators, and have a negative impact as pests. Insects have a wide habitat distribution, such as the surface of the ground or underground, fresh water, air, mud, remains of organisms, and parasites on various plants and animals. The presence of insects can be influenced by environmental conditions as their habitat. Therefore, this study aims to determine the diversity of insects in the Dramaga Protected Forest. The study was conducted in the Dramaga Protected Forest with four research methods, namely Pitfall Trap, Sweep Net, Yellow Sticky Trap and Active Searching. Insect catch data were analyzed for diversity index, evenness index and dominance index. The results of this study were a total of 1359 insect individuals consisting of 14 families. The diversity of insects with the four research methods was relatively low because there were dominant families, namely Formicidae, Acrididae, Blattelidae and Cicadellidae. It can be concluded that the determination of insect diversity is more effective using the Sweep Net method. The results of abiotic measurements at the research location showed optimal conditions so that the Dramaga Protected Forest has a good habitat for insect life.